Optimal assembly for high throughput shotgun sequencing

High throughput sequencing technologies — an algorithm for genome assembly

s 44 2010

Shotgun protein sequencing with meta-contig assembly.

Full-length de novo sequencing from tandem mass (MS/MS) spectra of unknown proteins such as antibodies or proteins from organisms with unsequenced genomes remains a challenging open problem. Conventional algorithms designed to individually sequence each MS/MS spectrum are limited by incomplete peptide fragmentation or low signal to noise ratios and tend to result in short de novo sequences at l...

Transcript assembly and abundance estimation with high-throughput RNA sequencing

Title of dissertation: TRANSCRIPT ASSEMBLY AND ABUNDANCE ESTIMATION WITH HIGH-THROUGHPUT RNA SEQUENCING Bruce C. Trapnell, Jr., Doctor of Philosophy, 2010 Dissertation directed by: Professor Steven Salzberg Department of Computer Science We present algorithms and statistical methods for the reconstruction and abundance estimation of transcript sequences from high throughput RNA sequencing (“RNA...

High-throughput sequencing technologies.

The human genome sequence has profoundly altered our understanding of biology, human diversity, and disease. The path from the first draft sequence to our nascent era of personal genomes and genomic medicine has been made possible only because of the extraordinary advancements in DNA sequencing technologies over the past 10 years. Here, we discuss commonly used high-throughput sequencing platfo...

The Principles of Shotgun Sequencing and Automated Fragment Assembly